The insertion/deletion in the DNA-binding region allows the discrimination and subsequent identification of the glucocorticoid receptor 1 (gr1) and gr2 nucleotide sequences in gilthead sea bream (Sparus aurata): Standardizing the gr nomenclature for a better understanding of the stress response in teleost fish species

Cortisol carries out its physiological mechanism of action through the recognition by mineralocorticoid receptor (MR) and glucocorticoid (GR) 1 (GR1) GR2. Previous studies reported that main difference between gr1 gr2 nucleotide sequences resides in a 27-nucleotide insertion/deletion DNA-binding region, respectively. However, gilthead sea bream ( Sparus aurata ) annotation for seems contradictory. The sequence possesses characteristic insertion that, fact, is associated with sequence. Thus, this study aimed to elucidate bream. Clustal Omega alignment different fish species corroborated presence such region , Then, we design specific primers set amplification polymerase chain reaction (PCR). Importantly, partial has high similarity other already published species, being present all them region. We also detected European bass had not been named according criteria our makes an urgent call scientific community discuss establishment updated agreement allows homogenizing nomenclature defining better understanding stress response teleost species.